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    简便敏感的环介导等温扩增基因诊断新技术.pdf

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    简便敏感的环介导等温扩增基因诊断新技术.pdf

    !“#$: 400038 %34?A9BCD, EFGH: flydustyxb yahoo. com. cn ·IJKLM· 简便敏感的环介导等温扩增基因诊断新技术 !“ #$% %plm, ¨Y“:9?Z LAMP h iZ?lmR 1. 715 ? 2 ? LAMP h iv?, ¢w*£?Z?9?; h, c?: 9?Z LAMP hi?, ?:?。c:9 ?Z LAMP hi?¶?, ?hi”。 t£S“ LAMP Z?-?, ·?aZ?¡ ?, Y 3 5 ?-?Aa?»*?, ¨ Y 1 2 ?-?, ?B”» 75. 0% 94. 4%, ¨wCD ?56Z_?。E?1F81 DNA ?J?G? 1 h, _4Z-?X?»HY?3øZI?JKL |L?。T?, LAMP ?£?a+Zi Y?M?34?ZNO;P。 (、 LAMP ZLM 1. _4Z-?: ? LAMP Zhi?C, QROS?T /F7e3øQROTI?JK4, ?_47? ?。Mori ? 15HYtu?´¢£ LAMP _4-? ·267·CF-.*,GH 2005 ? 7 I 28 J 7 K Chin J Lab Med, July 2005,Vol 28,No. 7 , q4?RU:øI?JK?£?*VhiRU? 3, Bq?hi?Z?-?_4ZL?wV, L| ?£y-?。X¤ PCR hi?Bw_3QROSRO S/F, ?y?, a?4Z?JKL|_4-?, WW ?XY?TfL|-?, ¨c?whi?¶“? , DZ?xø?PQ。 2. ? LAMP: ?8 LAMP hiZ?, Nagamine ? 16Z? 2 ?C4ß LAMP Zhi, C4BR Z?NS7Gk, 89grs DNA Zeø。?3 ?J4GkZS?w=?C4Gk。?J4 ?'C4vw?S7Gk, ?R?ZhiR? ?。C47eZ? F2 ? F1 m (R B2 ? B1 m) , q F1 » F2 ZXR B1 » B2 ZX7e。t A, ?ZS DNA “NJ4Gk, “NC4G k, ¨ß¢hi?。?YC4Z LAMP hi?$? Z LAMP hi?m?/, 8¢-?”?。 3. #g DNA Z?/: LAMP Z_4RpGZS7, ¨abZ_?.?#gZfi lm, ?qcq LAMP Z _4C?/#gZfi lm?“ LAMP ZiY?%?。 Nagamine ? 17?x LAMP Z_4? 65ºC Y TspRI f?a, =HY?Z4 5'-GACACTGCA-3'?Vb7? 3'Y)G k, ?YZ DNA def? LAMP hi?A? Bst DNA d ef, ?·?grsc?, rs 1 ?#g DNA, ?B CD¢fZYy, E?hid?e£Z?L|。 4. $ LAMP PQ: /u?, XY$Gk (FISH) A 3NfiY“?$Z-?, 8g?n?ÆC? ?$Z:h、 ?-?Z+?53%c?ij。7e$ $GkAZ$ PCR, ?”»8¢-?Z”? ?, ?B?nkZ?, cPQ_4lm?-?nox øZp?、 -?qZor!Y?-?C DNA def sTFZ?。?tutu?, Maruyama ? 18x LAMP ?$Gk7e, Y“-?v? stx2STZ E. coli O157: H7。t£N$ PCR r?, ·?¶r? Z?, CD?-?Zno, ?H“iYXY?L| ?-?£Zw?。?, _42?+yZfi lm5?h X%plm, tAZ?7?”»x#¢_4lm?-? K。?K, ?YZ DNA def?Fy?, ?Z?L?-?。 ?fl ?y, LAMP ?ZNOPQAr?, q? ?¶, 、 ”、 ?»PQfi lm。?R, ? 4´?Z PCR pG, ¨T?_4Zz?, ?# g DNA Z?/Qߢ?。?q, ?9B?ß?” Z?/, ?| LAMP hiZ?¶, q4Z*?N ?、 _ZlmZ+?、 S7Z+?L|wV, 8 PQ”?。? LAMP AZ?V?L, LAMP x?$UV、 STUV?q5?£ ?ª?NOZ?M, ¨ LAMP hi? PCR ? ?¥Z?, ?C?*?9BR?¿ø。 参考文献 1Notomi T, Okayama H, Masubuchi H, et al.Loop-mediated isothermal amplification of DNA. Nucleic Acids Res,2000,28 : E63. 2Whiting SH, Champoux JJ.Properties of strand displacement synthesis by Moloney murine leukemia virus reverse transcriptase: mechanistic implications. J Mol Biol, 1998, 278: 559-577. 3Poon LL,Leung CS, Tashiro M, et al. Rapid detection of the severe acute respiratory syndrome(SARS)coronavirus by a loop-mediated isothermal amplification assay. Clin Chem, 2004, 50: 1050-1052. 4Hong TC, Mai QL, Cuong DV, et al. Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol, 2004, 4: 1956-1961. 5Iwamoto T, Sonobe T, Hayashi K.Loop-mediated isothermal amplification for direct detection of mycobacterium tuberculosis complex,M. avium,and M. intracellulare in sputum samples. J Clin Microbiol, 2003, 41: 2616-2622. 6Wakabayashi T,Yamashita R,Kakita T,et al. Rapid and sensitive diagnosisofadenoviralkeratoconjunctivitisbyloop-mediated isothermal amplification( LAMP)method. Curr Eye Res,2004, 29: 219-24. 7Endo S, Komori T, Ricci G, et al.Detection of gp43of Paracoccidioidesbrasiliensisbytheloop-mediatedisothermal amplification(LAMP)method . FEMS Microbiol Lett,2004, 234: 93-97. 8Nagamine K,Watanabe K,Ohtsuka K,et al.Loop-mediated isothermal amplification reaction using a nondenatured template. Clin Chem, 2001, 47: 1742-1743. 9Poon LL,Leung CS,Chan KH,et al. Detection of human influenza avirusesbyloop-mediatedisothermalamplification.JClin Microbiol, 2005, 43: 427-430. 10Okamoto S,Yoshikawa T,Ihira M,et al.Rapid detection of varicella-zostervirusinfectionbyaloop-mediatedisothermal amplification method. J Med Virol, 2004, 74: 677-682. 11Horisaka T,Fujita K,Iwata T,et al.Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification. J Clin Microbiol, 2004, 42: 5349-5352. 12Yoshikawa T,Ihira M, Akimoto S,et al.Detection of human herpesvirus 7DNA by loop-mediated isothermal amplification. J Clin Microbiol, 2004, 42: 1348-1352. 13Kuboki N,Inoue N,Sakurai T,et al. Loop-mediated isothermal amplification for detection of african trypanosomes. J Clin Microbiol, 2003, 41: 5517 - 5524. 14Irayama H,Kageyama S,Moriyasu S ,et al. Rapid sexing of bovine preimplantation embryos using loop-mediated isothermal amplification. Theriogenology, 2004, 62: 887-896. 15Mori Y,Nagamine K,Tomita N,et al. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.Biochem Biophys Res Commun,2001, 289: 150-154. 16Nagamine K, Hase T, Notomi T, et al. Accelerated reaction by loop- mediated isothermal amplification using loop primers.Mol Cell Probes , 2002, 16: 223-229. 17Nagamine K,Kuzuhara Y,Notomi T,et al.Isolation of single- stranded DNA from loop-mediated isothermal amplification(LAMP) products. Biochem Biophys Res Commun, 2002, 290: 1195-1198. 18Maruyama F,Kenzaka T,Yamaguchi N,et al. Detection of Bacteria Carryingthestx2GenebyInSituLoop-MediatedIsothermal Amplification. Appl Envir Microbiol, 2003, 69: 5023-5028. (?K: 2004-07-19) (?: ?) ·367·CF-.*,GH 2005 ? 7 I 28 J 7 K Chin J Lab Med, July 2005,Vol 28,No. 7

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