GOOD27s缓冲液.doc

Buffer Reference CenterUseful pH Ranges of Selected Biological Buffers (25 C, 0.1M)BuffersUseful pH RangepKa(at 20)pKa(at 25)pKa(at 37)Reagent GradeBPCGradeUltraGradeMES5.56.76.166.105.97M8250M2933M5287Bis-Tris5.87.2n/a6.506.36B9754B4429B7535ADA6.07.26.656.596.46A9883n/aA8074aces6.17.56.886.786.54A9758A3594A7949PIPES6.17.56.806.766.66P6757P1851P8203MOPSO6.27.6n/a6.906.75M8389n/an/aBis-Tris Propane6.39.5n/a6.8, 9.0n/aB6755B4679B9410BES6.47.87.177.096.90B9879B4554B6420MOPS6.57.97.287.207.02M1254M3183M5162TES6.88.27.507.407.16T1375T5691T6541HEPES6.88.27.557.487.31H3375H4034H7273DIPSO7.08.2n/a7.607.35D9648n/aD0306MOBS6.98.3n/a7.60n/aM3295n/an/aTAPSO7.08.2n/a7.607.39T9269T5566T0432Trizma7.09.08.208.067.72T1503T6066T6791HEPPSO7.18.5n/a7.806.66H3137n/an/aPOPSO7.28.5n/a7.807.63P3405n/aP7088TEA7.38.3n/a7.80n/aT1377n/an/aEPPS7.38.7n/a8.00n/aE9502E0276E1894Tricine7.48.88.168.057.80T0377T5816T9784Gly-Gly7.58.9n/a8.20n/aG1002G3915G7278Bicine7.69.08.358.268.04B3876n/aB8660HEPBS7.69.0n/a8.30n/aH6903n/an/aTAPS7.7-9.18.498.408.18T5130T5316T9659AMPD7.89.7n/a8.80n/aA9754n/aA9074TABS8.29.6n/a8.90n/aT1302n/an/aAMPSO8.39.7n/a9.009.10A6659n/aA7585CHES8.610.09.559.499.36C2885n/aC8210CAPSO8.910.3n/a9.609.43C2278n/aC8085AMP9.010.5n/a9.70n/aA9879n/aA9199CAPS9.711.110.5610.4010.02C2632n/aC6070CABS10.011.4n/a10.70n/aC5580n/an/aTrizma Buffer Table pH vs. Temperature（三羟甲基氨基甲烷）pH at Temperatureg/L for 0.05 M Solution5 C25 C37 CTrizma HCLTrizma Base7.767.206.917.020.677.897.307.026.850.807.977.407.126.610.978.077.507.226.351.188.187.607.306.061.398.267.707.405.721.668.377.807.525.321.978.487.907.624.882.308.588.007.714.442.658.688.107.804.022.978.788.207.913.543.348.888.308.013.073.708.988.408.102.644.039.098.508.222.214.369.188.608.311.834.659.288.708.421.504.909.368.808.511.235.139.478.908.620.965.329.569.008.700.765.47Phosphate Buffer Table 0.2M solution（磷酸盐缓冲液）PotassiumPhosphate Monobasic Anhydrous g/LSodium PhosphateDibasicHeptahydrate g/L23 C pHPotassium Phosphate Monobasic Anhydrous g/LSodium PhosphateDibasic Heptahydrate g/L23 C pH22.43.495.710.8029.516.922.084.295.89.3632.737.021.605.375.97.9235.957.121.056.606.06.7238.637.220.408.056.15.5241.317.319.569.936.24.5643.467.418.6012.076.33.8445.077.517.6414.226.43.1246.687.616.4416.906.52.5248.557.715.0020.126.62.0449.097.813.5623.346.71.6849.897.912.2426.296.81.2750.818.0Citric Acid Na2HPO4 Buffer Solutions, pH approx. 2.67.6Citric acid monohydrate, C6H8O7 H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.40 g/l, or Na2HPO4 2H2O, M. wt. 178.05; 0.2M-solution contains 35.61 g/l.x ml 0.1M-citric acid and y ml 0.2M-Na2HPO4mixed.pHx ml 0.1M-citric acidy ml 0.2-Na2HPO42.689.1010.902.884.1515.853.079.4520.553.275.3024.703.471.5028.503.667.8032.203.864.5035.504.061.4538.554.258.6041.404.455.9044.104.653.2546.754.850.7049.305.048.5051.505.246.4053.605.444.2555.755.642.0058.005.839.5560.456.036.8563.156.233.9066.106.430.7569.256.627.2572.756.822.7577.257.017.6582.357.213.0586.957.49.1590.857.66.3593.65Citric Acid Sodium Citrate Buffer Solutions, pH 3.06.2Citric acid monohydrate, C6H8O7 H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Trisodium citrate dihydrate, C6H5O7Na3 2H2O, M. wt. 294.12; 0.1M-solution contains 29.41 g/l.x ml 0.1M-citric acid and y ml 0.1M-trisodium citrate mixed.pHx ml 0.1 M-citric acidy ml 0.1M-trisodium citrate3.082.018.03.277.522.53.473.027.03.668.531.53.863.536.54.059.041.04.254.046.04.449.550.54.644.555.54.840.060.05.035.065.05.230.569.55.425.574.55.621.079.05.816.084.06.011.588.56.28.092.0Sodium Acetate Acetic Acid Buffer Solutions, pH 3.75.6Sodium acetate trihydrate, CH3COONa 3H2O, M. wt. 136.09; 0.2M-solution contains 27.22 g/l.x ml 0.2M-NaOAc and y ml 0.2M-HOAc mixed.pH, 18 Cx ml 0.2M-NaOAcy ml 0.2M-HOAc3.710.090.03.812.088.04.018.082.04.226.573.54.437.063.04.649.051.04.859.041.05.070.030.05.279.021.05.486.014.05.691.09.0Na2HPO4 NaH2PO4 Buffer Solutions, pH 5.88.0 at 25 CNa2HPO4 2H2O, M. wt. 178.05; 0.2M-solution contains 35.61 g/l. Na2HPO4 12H2O, M. wt. 358.22; 0.2M -solution contains 71.64 g/l. NaH2PO4 H2O, M. wt. 138.01; 0.2M-solution contains 27.6 g/l. NaH2PO4 2H2O, M. wt. 156.03; 0.2M-solution contains 31.21 g/l.x ml 0.2M-Na2HPO4, y ml 0.2M-NaH2PO4; diluted to 100 ml with H2O.pH, 25 Cx ml 0.2M-Na2HPO4y ml 0.2M-NaH2PO45.84.046.06.06.1543.856.29.2540.756.413.2536.756.618.7531.256.824.525.57.030.519.57.236.014.07.440.59.57.643.56.57.845.754.258.047.352.65Imidazole (glyoxaline) HCl buffer solutions, pH 6.27.8 at 25 C1Imidazole, C3H4N2, M. wt. 68.08.25 ml 0.2M-imidazole (13.62 g/l), x ml 0.2M-HCl, diluted to 100 ml with H2O.pH, 25 Cx ml 0.2M-HCl6.221.456.419.96.617.756.815.27.012.157.29.37.46.87.64.657.83.0Sodium Carbonate Sodium Bicarbonate Buffer Solutions, pH 9.210.81Na2CO3 10H2O, M. wt. 286.2; 0.1M-solution contains 28.62 g/l. NaHCO3, M. wt. 84.0; 0.1M-solution contains 8.40 g/l.x ml 0.1M-Na2CO3and y ml 0.1M-Na2HCO3mixed.pHx ml 0.1M-Na2CO3y ml 0.1M-NaHCO320 C37 C9.28.810909.49.120809.59.430709.89.540609.99.7505010.19.9604010.310.1703010.510.3802010.810.69010Helpful FormulasPercentage by weight (w/v)(% buffer desired / 100)xfinal buffer volume (ml) = g of starting material needed.Molar Solutionsdesired molarityxformula weightxsolution final volume (L) = grams neededHenderson-Hasselbach EquationReference1. Dawson, R. M. C.; Elliot, D. C.; Elliot, W. H.; Jones, K. M.Data for Biochemical Research;3rd ed., Oxford Science Publ., 1986.- See more at: http:/www.sigmaaldrich.com/china-mainland/zh/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#sthash.mIVwsb3Z.dpuf