基于GQDs的荧光猝灭检测抗坏血酸的新方法(4).docx
基于GQDs的荧光猝灭检测抗坏血酸的新方法(4)3. 7 特异性考察。为了考察所建立方法的特异性，实验选用几种糖类以及 HSA、尿素（ Urea） 和几种氨基酸作为对照样品进行分析。GQDs 的浓度为 0. 14 mg/mL,AA 的浓度为 7. 5 × 10- 5mol / L,其他作为干扰物质的浓度均为 7. 5 × 10- 5mol / L.实验结果如图 8 所示。从图中可以看出，只有 AA 的加入能引起信号的明显变化，其他物质均不存在干扰，特别是 DA、UA 这两种与 AA 有相似电化学性质的物质，也不存在干扰。因为在本实验中，AA 与GQDs 的反应介质为 pH = 4. 5 的磷酸盐缓冲液，在这个 pH 值下，DA、UA 均不干扰 AA 的测定。3. 8 实际样品分析。为了考察本研究所建立的方法是否可用于实际样品的检测，实验对人血清样品进行了分析。从医院取的血清样品按照 2. 2 节方法进行处理，在稀释 50 倍后的血清中进行加标实验，实验结果如表 3 所示。从表中可以看到，人血清中 AA 的加标回收率在 95. 2% 115. 3%范围内。4 结 论。建立了一种基于 GQDs 荧光猝灭检测 AA的新方法，该方法操作简便，灵敏度高，检出限低至 1. 0 ×10- 6mol / L,特异性强，一些糖类和氨基酸的存在均不干扰 AA 的测定，甚至是与 AA有相似电化学性质的 DA、UA 的存在对 AA 的检测也没有影响。本实验用到的所有原料都价廉易得且不需要任何修饰过程，GQDs 相对于其他量子点的合成更简单而且具有低毒性，有望应用于生物体内生物活性物质的检测。参 考 文 献：1PONOMARENKO L A,SCHEDIN F,KATSNELSON M I,et al. . Chaotic dirac billiard in graphene quantum dots J.Science,2008,320（ 5874） : 356-358.2PAN D Y,ZHANG J C,LI Z,et al. . Hydrothermal route for cutting graphene sheets into blue-luminescent graphenequantum dots J. Adv. 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