分子生物学(双语)3 Methods in Molecular Biology and Genetic Engineering.ppt
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1、Chapter 3 Methods in Molecular Biology and Genetic Engineering FIGURE CO: Methods in Molecular Biology and Genetic Engineering Nicemonkey/D FIGURE 01: The target of a phosphatase (a) and a nuclease (b). An endonuclease (c) and an exonuclease (d) FIGURE 02: Restriction endonuclease FIGURE 03: A restr
2、iction map is a linear sequence of sites separated by defined distances on DNA FIGURE 04: Plasmid transformation FIGURE 05: E. coli colonies on agar plates with ampicillin, IPTG, and the color indicator X-gal FIGURE 06: Several types of cloning vectors are available FIGURE 07: A vector can be used i
3、n both yeast and bacteria FIGURE 08: Luciferase graph/Firefly Photo Cathy Keifer/D FIGURE 09: A mouse promoter controls tissue-specific expression of lacZ Photo courtesy of Robb Krumlauf, Stowers Institute for Medical Research FIGURE 10A: Fluorescent proteins are powerful research tools Courtesy of
4、Joachim Goedhart, Molecular Cytology, SILS, University of Amsterdam. FIGURE 10B: Fluorescent proteins are powerful research tools Reprinted from Vision Res., vol. 45, T. G. Wensel, et al., Rhodopsin-EGFP knock-ins., pp. 3445-3453. Copyright 2005, with permission from Elsevier http:/ Photo courtesy o
5、f Theodore G. Wensel, Baylor Col FIGURE 11: DNA can be introduced into cells in several ways FIGURE 12: An autoradiogram of a gel prepared from the colonies described in Figure 3.5 FIGURE 13: The fluorescent in situ hybridization (FISH) technique Adapted from an illustration by Darryl Leja, National
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