AATCC 194-2006 纺织品在长期测试条件抗室内尘螨性能的测定1.pdf
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1、标准分享网 w w w . b z f x w . c o m 免费下载 Copyright 2006 American Association of Textile Chemists and Colorists AATCC Technical Manual/2007TM 194-2006375 Developed 2006 by AATCC Committee RA49. 1. Purpose and Scope 1.1 This test method is for the evalua- tion of the degree of anti-house dust mite activit
2、y in a long-term testing environ- ment for textiles treated at the manufac- turing level for this purpose. 2. Principle 2.1 Test and control specimens are in- oculated with the test organism and nutri- ents. After six weeks of incubation, suffi- cient time for mite colonies to flourish under optimal
3、 conditions, the dust mites are recovered from the specimens by heat extraction. Results are expressed as per cent reduction on the treated sample ver- sus the untreated control. 3. Terminology 3.1 activity, n.of an anti-dust mite agent, a measure of the effectiveness of the agent. 3.2 anti-house du
4、st mite agent, n. any chemical which kills (acaricide) or repels house dust mites. 4. Safety Precautions NOTE: These safety precautions are for information purposes only. The pre- cautions are ancillary to the testing proce- dure and are not intended to be all inclu- sive. It is the users responsibi
5、lity to use safe and proper techniques in handling materials in this test method. Manufac- turers MUST be consulted for specific details such as material safety data sheets and other manufacturers recommenda- tions. All OSHA standards and rules must also be consulted and followed. 4.1 This test shou
6、ld be carried out by persons with training and experience in the use of acarological techniques. 4.2 CAUTION: Although house dust mites are not considered to be a direct hazard to humans, their fecal pellets have been demonstrated to be a potential po- tent allergen for those susceptible to asthma.
7、Therefore, every necessary and reasonable precaution must be taken to eliminate this risk to the laboratory per- sonnel and to personnel in the associated environment. Where appropriate, wear protective clothing and respiratory pro- tection that prevents penetration by the matter. 4.3 All work shoul
8、d be conducted us- ing standard safe laboratory practices. 4.4 All chemicals should be handled with care. 4.5 An eyewash/safety shower should be located nearby for emergency use. 4.6 Exposure to chemicals used in this procedure must be controlled at or below levels set by government authorities (e.g
9、., Occupational Safety and Health Admin- istrations OSHA permissible exposure limits PEL as found in 29 CFR 1910.1000 of January 1, 1989). In addi- tion, the American Conference of Gov- ernmental Industrial Hygienists (ACGIH) Threshold Limit Values (TLVs) com- prised of time weighted averages (TLV-
10、TWA), short term exposure limits (TLV- STEL) and ceiling limits (TLV-C) are recommended as a general guide for air contaminant exposure which should be met (see 13.1). 5. Limitations 5.1 The method can not be used to de- termine the specific mode of action of a given acaricide treatment. 5.2 The met
11、hod should not be used to predict the performance of a finished arti- cle if the test specimen will go through additional processing steps or represents only a component of the final article. 5.3 While this test method provides some insight into the effect of different textile treatments in controll
12、ing the abil- ity of mites to successfully establish breeding colonies, direct conclusions can not be made with regard to a treatment re- moving or reducing allergens. 5.4 This test method does not allow recovery of house dust mite eggs. How- ever, it does provide a good measurement of the effective
13、ness of anti-dust mite treatment on a breeding mite colony. 6. Test Organisms 6.1 Test mites: Dermatophagoides pteronyssinus or D. farinae. Any other species suitable to a given country or re- gion can also be used. 7. Maintenance of House Dust Mite Stock Cultures 7.1 Dust mite colonies should be ma
14、in- tained at 25 1C (77 1F) and 73-76% relative humidity on one part desiccated ox liver powder: one part dried yeast powder (see 13.2). Before use, the mix- ture should first be ground with a mortar and pestle, then sieved so that the particle size is between 500750 m. 7.2 Care must be taken to ens
15、ure that stock cultures of mites used for testing have not previously been exposed to chemicals or treatments that might have some interaction with the mites. 8. Preparation of Specimens 8.1 Test Specimens. 8.1.1 Prepare a minimum of three test specimens by cutting them to fit snugly in the bottom o
16、f a 10 cm diameter glass or polystyrene Petri dish. This can accu- rately be done by tracing around the dish on the sample, or by using of a suitably sized die. For loose fibers, sufficient ma- terial to cover the bottom of the Petri dish should be used. 8.1.2 Petri dishes or other test cham- bers o
17、f larger or smaller size can be sub- stituted where desired. The size of the test specimen should change accordingly to ensure a snug fit of the sample in the bottom of the dish. 8.2 Control specimens. 8.2.1 A minimum of three specimens of the same fiber type and construction as the test specimen bu
18、t containing no anti- mite finish (negative control) should also be prepared. 8.2.2 In addition, an internal lab con- trol previously determined to support dust mite colonies should be included for each test. The purpose of this internal control is to provide validation that the mite colony has deve
19、loped at the ex- pected rate on a known sample over the course of the six weeks test. 8.3 Sterilization of Specimens. 8.3.1 Specimens may be sterilized when there is potential for fungal growth on the specimen over the test period due to the presence of spores. The method of sterilization used will
20、depend on the sam- ple composition and finish, as well as the particular anti-house dust mite treatment. Report method of sterilization, if used. 9. Procedure 9.1 Test Setup. 9.1.1 Distribute 50 mg of the ground/ sieved nutrient mixture on each test specimen. The mixture can be applied through a sie
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- AATCC 194-2006 纺织品在长期测试条件抗室内尘螨性能的测定1 194 2006 纺织品 长期 测试 条件 室内 性能 测定
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